Glossary entry (derived from question below)
English term or phrase:
rescue, rescue arm
Russian translation:
сохранение / линия клеток с сохраненным/введенным геном [таким-то]
Added to glossary by
Natalie
- The asker opted for community grading. The question was closed on 2018-06-25 16:54:08 based on peer agreement (or, if there were too few peer comments, asker preference.)
Jun 22, 2018 12:17
5 yrs ago
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English term
rescue, rescue arm
English to Russian
Medical
Genetics
Percent growth of HCT116 wt and HCT116 MTAP-/- cells upon PRMT5 knockdown (+dox), with or without PRMT5 wt or R368A mutant rescue.
PRMT5 knockdown with doxycycline-inducible shRNA led to more complete growth reduction in cells with MTAP deletion than in MTAP WT cells. Expression of an shPRMT5-resistant PRMT5 cDNA in the MTAP-null cells rescued growth inhibition upon endogenous PRMT5 knockdown, while expression of catalytically-dead R368A PRMT5 mutant cDNA did not.
we performed expanded in vivo study with a wild type MAT2A rescue arm of shMAT2A; growth inhibition was rescued in the xenograft expressing a MAT2A cDNA that was resistant to the MAT2A shRNA.
PRMT5 knockdown with doxycycline-inducible shRNA led to more complete growth reduction in cells with MTAP deletion than in MTAP WT cells. Expression of an shPRMT5-resistant PRMT5 cDNA in the MTAP-null cells rescued growth inhibition upon endogenous PRMT5 knockdown, while expression of catalytically-dead R368A PRMT5 mutant cDNA did not.
we performed expanded in vivo study with a wild type MAT2A rescue arm of shMAT2A; growth inhibition was rescued in the xenograft expressing a MAT2A cDNA that was resistant to the MAT2A shRNA.
Proposed translations
(Russian)
| 4 | сохранение / линия клеток с сохраненным геном [таким-то] |
Natalie
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Change log
Jun 25, 2018 18:22: Natalie Created KOG entry
Proposed translations
3 hrs
Selected
сохранение / линия клеток с сохраненным геном [таким-то]
Например, wild type MAT2A rescue arm of shMAT2A - линия shMAT2A с сохраненным геном MAT2A дикого типа
4 KudoZ points awarded for this answer.
Comment: "Natalie, спасибо большое за помощь!"
Discussion
You can rescue the phenotype of the gene by over-expression of a cDNA or introducing large amounts of protein into the cell to out-compete the knockdown.
http://dharmacon.horizondiscovery.com/resources/faqs/how-res...
Вместо внесения может быть и индуцированная сверхэкспрессия того же гена
Rescue experiments are always tricky but I'd be concerned about reviewing something which suddenly switched to an overexpressing line with transient knockdown when you have a stable knockdown already available which you simply need to re-express your protein in.
https://www.researchgate.net/post/Can_anyone_help_me_regardi...
https://www.researchgate.net/post/Rescue_Experiment_to_exclu...
The rescue arm of knock-down and add back experiments would
be most simply performed...
Не дочитал )
там помощь скорее относится к donation
Вот нашла еще, что народ употребляет в аналогичных случаях слово "помощь":
plasmid donation (rescue), homologous drift - плазмидная “помощь”. Процесс перемещения неконъюгативных плазмид <non-conjugative plasmid> из клетки-донора в клетку-реципиент...
http://wsab-forest.sbras.ru/attachments/article/19/anglrusbi...
Rescue arm - группа "помощи"? :-)
Но не согласен с "сохранением гена".
И речь именно о контрольном эксперименте, подобном описанному на сайте термофишера, я привел ссылку на патент, краткая схема описана в легенде к рис. 5
Или здесь, например: http://www.oxfordreference.com/view/10.1093/oi/authority.201...
The introduced wild-type gene sequences are said to “rescue” the mutant phenotype to normality.
The rescue arm of knock-down and add back experiments would
be most simply performed by introducing expression vectors
encoding cDNA with silent mutations preventing cleavage by
Cas9–sgRNA.
https://www.horizondiscovery.com/media/resources/Publication...
shMAT2A это short hairpine RNA MAT2A, используемая для подавления экспрессии MAT2A и экспрессируемая клетками
wild type MAT2A rescue arm of shMAT2A - это вероятнее всего MAT2A cDNA that was resistant to the MAT2A shRNA (или клетки которые ее продуцируют), которая использована для проведения rescue experiment, необходимого, чтобы показать, что именно подавление экспрессии MAT2A приводит к прекращению деления клеток (growth inhibition)
https://patents.google.com/patent/WO2017096165A1/en
суть rescue experiment см. тут п.1
https://www.thermofisher.com/us/en/home/references/ambion-te...
Но на ум пока особо ничего гениального не приходит, возможно Вам приходилось сталкиваться
Простите, если несколько сумбурно к ночи